Post Doctorate Fellow, Identifying cardiac disease pathways using pooled CRISPR screens in iPSC-derived cardiomyocytes

Ionis is seeking a highly motivated and productive Postdoctoral Fellow to lead a project applying single-cell genomics and high throughput pooled perturbation screens to identify novel therapeutic targets for heart failure. The successful candidate will work with internal team members and external partners to design, implement and analyze pooled genetic screens in cardiac cells to assign phenotypes to genes with previously unknown functions and prioritize targets within biological pathways of interest.

Requirements

• Ph.D. in RNA biology, genetics, molecular biology, bioengineering, or a closely related field with 0-3 years
• Experience and/or familiarity with CRISPR-Cas9 genetic perturbations, pooled screening, RNA/DNA-sequencing and single cell RNA-seq and supporting bioinformatics tools
• Cell culture experience including immortalized and iPSC-derived or primary cells
• In vivo rodent disease model experience or willingness to learn
• Familiarity with molecular techniques (e.g., plasmid design/cloning, RNA/DNA isolation, qPCR, NGS library construction)
• Excellent problem-solving skills and ability to design experiments independently
• Ability to work effectively in a matrixed, multidisciplinary team environment
• Strong written and verbal communication skills for publication and presentation
• Ability or familiarity with tools to analyze large datasets (e.g., scRNA-seq, transcriptomics, pooled CRISPR screens with R/Python using Jupyter notebooks) is a plus
• Strong background in iPSC cardiomyocyte differentiation, culture optimization, phenotypic assays or cardiovascular physiology is a plus

Benefits

• Competitive salary
• Health and dental insurance
• Relocation compensation